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cd3 t cell fraction  (Miltenyi Biotec)


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    Miltenyi Biotec cd3 t cell fraction
    Cd3 T Cell Fraction, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd3+t+cell+fractions/CD3%CE%B5+Antibody%2C+anti-mouse%2C+REAdye_lease/pmc10692662-337-4-30
    Average 99 stars, based on 5 article reviews
    cd3 t cell fraction - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Very early lineage-specific chimerism after reduced intensity stem cell transplantation is highly predictive of clinical outcome for patients with myeloid disease.
    Article Snippet: .. Analyses were undertaken on whole blood derived PBMC and on purified CD3+ T-cell fractions, which were separated from PBMCs using magnetic bead selection (Miltenyi Biotec). ..

    Purification:

    Article Title: Very early lineage-specific chimerism after reduced intensity stem cell transplantation is highly predictive of clinical outcome for patients with myeloid disease.
    Article Snippet: .. Analyses were undertaken on whole blood derived PBMC and on purified CD3+ T-cell fractions, which were separated from PBMCs using magnetic bead selection (Miltenyi Biotec). ..

    Selection:

    Article Title: Very early lineage-specific chimerism after reduced intensity stem cell transplantation is highly predictive of clinical outcome for patients with myeloid disease.
    Article Snippet: .. Analyses were undertaken on whole blood derived PBMC and on purified CD3+ T-cell fractions, which were separated from PBMCs using magnetic bead selection (Miltenyi Biotec). ..

    Isolation:

    Article Title: CCR7 + selected gene-modified T cells maintain a central memory phenotype and display enhanced persistence in peripheral blood in vivo
    Article Snippet: .. CCR7 + and CD62L + T cells were isolated from enriched CD3+ T cell fractions using clones FR11-11E8 (Miltenyi Biotec, Germany) and DREG-56 (eBioscence, USA) respectively; both of which were conjugated to APC fluorophore. .. MACS separation based on APC (Miltenyi Biotec, Germany) was used to isolate CCR7 + or CD62L + cells with negative fractions were collected as effluent.

    Clone Assay:

    Article Title: CCR7 + selected gene-modified T cells maintain a central memory phenotype and display enhanced persistence in peripheral blood in vivo
    Article Snippet: .. CCR7 + and CD62L + T cells were isolated from enriched CD3+ T cell fractions using clones FR11-11E8 (Miltenyi Biotec, Germany) and DREG-56 (eBioscence, USA) respectively; both of which were conjugated to APC fluorophore. .. MACS separation based on APC (Miltenyi Biotec, Germany) was used to isolate CCR7 + or CD62L + cells with negative fractions were collected as effluent.



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    ( A ) Purified <t>CD3</t> + <t>CD4</t> + T cells were labeled with CFSE and cultured as described in ± TMV or DC-derived MV (5 µg/mL). On days 3, 5 and 8, the frequency of CD4 + CD25 + FOXP3 + Treg among proliferating T cells was determined by flow cytometry. The data (means ± SD) represent three independent experiments (*p<0.01). ( B ) Proliferating CD3 + CD4 + T cells (squares) were tested for co-expression of CD25 in a representative co-culture ± TMV. A higher proportion of proliferating CD4 + T cells expressed CD25 in the co-culture with TMV than without TMV. ( C ) The proliferating CD4 + CD25 + T cells in the co-cultures with TMV were evaluated for the frequency of FOXP3 + T cells upon gating on the CD4 + CD25 high subset (see box). Over 90% of these cells also expressed intracellular FOXP3. Data are representative for one out of 6 cultures tested.
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    Image Search Results


    ( A ) Purified CD3 + CD4 + T cells were labeled with CFSE and cultured as described in ± TMV or DC-derived MV (5 µg/mL). On days 3, 5 and 8, the frequency of CD4 + CD25 + FOXP3 + Treg among proliferating T cells was determined by flow cytometry. The data (means ± SD) represent three independent experiments (*p<0.01). ( B ) Proliferating CD3 + CD4 + T cells (squares) were tested for co-expression of CD25 in a representative co-culture ± TMV. A higher proportion of proliferating CD4 + T cells expressed CD25 in the co-culture with TMV than without TMV. ( C ) The proliferating CD4 + CD25 + T cells in the co-cultures with TMV were evaluated for the frequency of FOXP3 + T cells upon gating on the CD4 + CD25 high subset (see box). Over 90% of these cells also expressed intracellular FOXP3. Data are representative for one out of 6 cultures tested.

    Journal: PLoS ONE

    Article Title: Tumor-Derived Microvesicles Induce, Expand and Up-Regulate Biological Activities of Human Regulatory T Cells (Treg)

    doi: 10.1371/journal.pone.0011469

    Figure Lengend Snippet: ( A ) Purified CD3 + CD4 + T cells were labeled with CFSE and cultured as described in ± TMV or DC-derived MV (5 µg/mL). On days 3, 5 and 8, the frequency of CD4 + CD25 + FOXP3 + Treg among proliferating T cells was determined by flow cytometry. The data (means ± SD) represent three independent experiments (*p<0.01). ( B ) Proliferating CD3 + CD4 + T cells (squares) were tested for co-expression of CD25 in a representative co-culture ± TMV. A higher proportion of proliferating CD4 + T cells expressed CD25 in the co-culture with TMV than without TMV. ( C ) The proliferating CD4 + CD25 + T cells in the co-cultures with TMV were evaluated for the frequency of FOXP3 + T cells upon gating on the CD4 + CD25 high subset (see box). Over 90% of these cells also expressed intracellular FOXP3. Data are representative for one out of 6 cultures tested.

    Article Snippet: Total CD3 + CD4 + T cell fractions or isolated CD4 + CD25 + cells were cultured in AIMV medium with plate-bound OKT3 (1 µg/mL; American Type Culture Collection), soluble anti-CD28 Abs (1 µg/mL) and IL-2 (150 IU/mL) at 37°C/5%CO 2 in wells of 96-wells plates.

    Techniques: Purification, Labeling, Cell Culture, Derivative Assay, Flow Cytometry, Expressing, Co-Culture Assay

    ( A ) Flow cytometry analysis of TGF-β1 and IL-10 expression on TMV purified from OVCAR-3 SN and coated onto latex beads. ( B ) CD4 + CD25 high FOXP3 + T cells were cultured with OKT3, anti-CD28 and IL-2 (150 IU/mL) +/− TMV for 72 h at 37°C in the presence of Golgistop and then stained for CD4, CD3, CD25 and intracellular TGF-β1 and IL-10. Expression of both cytokines was up-regulated in the presence of TMV (p<0.05). ( C ) SMAD2/3 and STAT3 phosphorylation in Treg before and after exposure to TMV. Representative results are from one of three independent experiments for A , B and C . ( D ) The percentage of CD4 + CD25 high FOXP3 + T cells increased among CD4 + CD25 + T cells cultured in the presence of TMV but not DC-derived MV. Neutralizing anti-TGF-β1 and/or anti-IL-10 Abs inhibited the induction of Treg by TMV. Non-blocking IgG isotype control Abs were used as controls. Asterisks indicate decreases (p<0.05) in Treg percentages in the presence of neutralizing Abs. Results are means ± SD of three independent experiments.

    Journal: PLoS ONE

    Article Title: Tumor-Derived Microvesicles Induce, Expand and Up-Regulate Biological Activities of Human Regulatory T Cells (Treg)

    doi: 10.1371/journal.pone.0011469

    Figure Lengend Snippet: ( A ) Flow cytometry analysis of TGF-β1 and IL-10 expression on TMV purified from OVCAR-3 SN and coated onto latex beads. ( B ) CD4 + CD25 high FOXP3 + T cells were cultured with OKT3, anti-CD28 and IL-2 (150 IU/mL) +/− TMV for 72 h at 37°C in the presence of Golgistop and then stained for CD4, CD3, CD25 and intracellular TGF-β1 and IL-10. Expression of both cytokines was up-regulated in the presence of TMV (p<0.05). ( C ) SMAD2/3 and STAT3 phosphorylation in Treg before and after exposure to TMV. Representative results are from one of three independent experiments for A , B and C . ( D ) The percentage of CD4 + CD25 high FOXP3 + T cells increased among CD4 + CD25 + T cells cultured in the presence of TMV but not DC-derived MV. Neutralizing anti-TGF-β1 and/or anti-IL-10 Abs inhibited the induction of Treg by TMV. Non-blocking IgG isotype control Abs were used as controls. Asterisks indicate decreases (p<0.05) in Treg percentages in the presence of neutralizing Abs. Results are means ± SD of three independent experiments.

    Article Snippet: Total CD3 + CD4 + T cell fractions or isolated CD4 + CD25 + cells were cultured in AIMV medium with plate-bound OKT3 (1 µg/mL; American Type Culture Collection), soluble anti-CD28 Abs (1 µg/mL) and IL-2 (150 IU/mL) at 37°C/5%CO 2 in wells of 96-wells plates.

    Techniques: Flow Cytometry, Expressing, Purification, Cell Culture, Staining, Derivative Assay, Blocking Assay